Peptide Level Immunoaffinity Enrichment Enhances Ubiquitination Site Identification on Individual Proteins
نویسندگان
چکیده
منابع مشابه
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
There is a great need for quantitative assays in measuring proteins. Traditional sandwich immunoassays, largely considered the gold standard in quantitation, are associated with a high cost, long lead time, and are fraught with drawbacks (e.g. heterophilic antibodies, autoantibody interference, 'hook-effect').(1) An alternative technique is affinity enrichment of peptides coupled with quantitat...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2014
ISSN: 1535-9476
DOI: 10.1074/mcp.m113.031062